Nature Protocols 10, 1098 (2015)

Guidelines for the automated evaluation of Elispot assays

Sylvia Janetzki, Leah Price, Helene Schroeder, Cedrik M Britten, Marij J P Welters, Axel Hoos.
This protocol summarizes the recommendations developed after a comparison of Elispot plate-reading approaches by a panel of laboratories and subsequent agreement regarding the optimal steps to follow when evaluating Elispot plates.