PNAS

Structural basis of a Ni acquisition cycle for [NiFe] hydrogenase by Ni-metallochaperone HypA and its enhancer [Biochemistry]

S. Watanabe, T. Kawashima, Y. Nishitani, T. Kanai, T. Wada, K. Inaba, H. Atomi, T. Imanaka, K. Miki.
The Ni atom at the catalytic center of [NiFe] hydrogenases is incorporated by a Ni-metallochaperone, HypA, and a GTPase/ATPase, HypB. We report the crystal structures of the transient complex formed between HypA and ATPase-type HypB (HypBAT) with Ni ions. Transient association between HypA and HypBAT is controlled by the ATP...