PNAS

Conformational processing of oncogenic v-Src kinase by the molecular chaperone Hsp90 [Biochemistry]

E. E. Boczek, L. G. Reefschlager, M. Dehling, T. J. Struller, E. Hausler, A. Seidl, V. R. I. Kaila, J. Buchner.
Hsp90 is a molecular chaperone involved in the activation of numerous client proteins, including many kinases. The most stringent kinase client is the oncogenic kinase v-Src. To elucidate how Hsp90 chaperones kinases, we reconstituted v-Src kinase chaperoning in vitro and show that its activation is ATP-dependent, with the cochaperone Cdc37...