Motivation: Proteogenomics has been well accepted as a tool to discover novel genes. In most conventional proteogenomic studies, a global false discovery rate (FDR) is used to filter out false positives for identifying credible novel peptides. However, it has been found that the actual level of false positives in novel peptides is often out of control and behaves differently for different genomes. In order to quantitatively model this problem, we theoretically analyze the sub-group FDRs of annotated and novel peptides. Our analysis shows that the annotation completeness ratio of a genome is the dominant factor influencing the subgroup FDR of novel peptides. Experimental results on two real datasets of E. coli and M. tuberculosis support our conjecture.

Contact: yfu@amss.ac.cn, xupingghy@gmail.com, smhe@ict.ac.cn