Nature Protocols 10, 974 (2015)

Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis

Yun Kang, Ian McMillan, Michael H Norris, Tung T Hoang.
This protocol enables amplification of the total transcript of a single prokaryotic cell for in-depth analysis. Laser-capture microdissection is used to isolate single cells, and amplified cDNA can be further analyzed by microarray.