PNAS

Random coil negative control reproduces the discrepancy between scattering and FRET measurements of denatured protein dimensions [Biophysics and Computational Biology]

H. M. Watkins, A. J. Simon, T. R. Sosnick, E. A. Lipman, R. P. Hjelm, K. W. Plaxco.
Small-angle scattering studies generally indicate that the dimensions of unfolded single-domain proteins are independent (to within experimental uncertainty of a few percent) of denaturant concentration. In contrast, single-molecule FRET (smFRET) studies invariably suggest that protein unfolded states contract significantly as the denaturant concentration falls from high (∼6 M) to low...