Nature Methods
Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR
Mohammed Kaplan, Abhishek Cukkemane, Gydo C P van Zundert, Siddarth Narasimhan, Mark Daniëls, Deni Mance, Gabriel Waksman, Alexandre M J J Bonvin, Rémi Fronzes, Gert E Folkers, Marc Baldus.
Dynamic nuclear polarization–based solid-state nuclear magnetic resonance spectroscopy is applied to study the structure of a large bacterial membrane protein complex in its cellular environment.