Nature Methods
A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy
Dhermendra K Tiwari, Yoshiyuki Arai, Masahito Yamanaka, Tomoki Matsuda, Masakazu Agetsuma, Masahiro Nakano, Katsumasa Fujita, Takeharu Nagai.
Kohinoor is a fast- and positively switching fluorescent protein with high photostability over many photoswitching cycles. Its improved photophysical properties enable nanoscopy with low phototoxicity and allow use of a simplified RESOLFT setup.